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KMID : 0624620120450120713
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BMB Reports
2012 Volume.45 No. 12 p.713 ~ p.718
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Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells
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Ryu Jae-Sung
Chang Kyu-Tae
Lee Ju-Taek
Lim Malg-Um
Min Hyun-Ki
Na Yoon-Ju
Lee Su-Bin
Moussavou Gislain
Kim Sun-Uk
Kim Ji-Su
Ko Ki-Narm
Ko Ki-Sung
Hwang Kyung-A
Jeong Eun-Jeong
Lee Jeong-Woong
Choo Young-Kug
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Abstract
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Gangliosides play important roles in the control of several biological processes, including proliferation and transmembrane signaling. In this study, we demonstrate the effect of ganglioside GM1 on the proliferation of mouse induced pluripotent stem cells (miPSCs). The proliferation rate of miPSCs was lower than in mouse embryonic stem cells (mESCs). Fluorescence activated cell sorting analysis showed that the percentage of cells in the G2/M phase in miPSCs was lower than that in mESCs. GM1 was expressed in mESCs, but not miPSCs. To confirm the role of GM1 in miPSC proliferation, miPSCs were treated with GM1. GM1-treated miPSCs exhibited increased cell proliferation and a larger number of cells in the G2/M phase. Furthermore, phosphorylation of mitogen-activated protein kinases was increased in GM1- treated miPSCs.
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KEYWORD
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Cell cycle, Ganglioside GM1, MAP kinase, Mouse induced pluripotent stem cells (miPSCs), Proliferation
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